Reverse correlation of bacterial density and surface expression of GFP and CD4 molecules. The pWZ486 transformant strain A486 in different initial concentrations (from high to low) is designated A486-1, A486-2, A486-3, and A486-4. The GFP and CD4 surface expression was evaluated by fluorescence-activated cell sorter (FACS), and the data are shown in the lower panel. CD4% is shown in red, and GFP% in green.