FIG 3.

Dynamin is required for JCPyV infection. (A) SVG-A cells were pretreated with the dynamin 1/2 inhibitor Dynole at the indicated concentrations or with the Dynole-specific negative control at 37°C for 30 min before infection with JCPyV (MOI = 0.5 FFU/cell) in the presence of Dynole. (B) SVG-A cells transfected with siRNA targeting the EGFR control, the nontargeting scrambled (Scr) control, dynamin 1 (Dyn1), or dynamin 2 (Dyn2) were infected with JCPyV (MOI = 0.5 FFU/cell). Infected cells were fixed at 72 hpi, stained using a VP1-specific antibody, and analyzed by indirect immunofluorescence. (C) Protein knockdown of Dyn1 and Dyn2 was confirmed by Western blotting. Data represent the percentages of VP1⁺ cells/visual field normalized to the number of DAPI⁺ cells/field for five ×10 fields of view for triplicate samples (values for all samples were normalized to that for EGFR control siRNA-treated cells [100%]) from at least three independent experiments. Error bars indicate SD. *, P < 0.05.