FIG 9.

Model of JCPyV entry. JCPyV initially interacts with LSTc via direct interactions with VP1. Internalization of JCPyV is facilitated by 5-HT₂Rs. 5-HT₂R-mediated entry likely first activates β-arrestin at the site of infection, facilitating an interaction with the receptor through the conserved 5-HT₂R ASK motif. Clathrin and AP2 subunits β2 and μ2 are recruited, likely forming a clathrin-coated pit around the viral cargo. Internalization is completed by the GTPase scission mechanism of dynamins 1 and 2, allowing for JCPyV to traffic through the endocytic compartment in a clathrin-coated vesicle.