FIG 3.

HCV-specific regulation of the viral life cycle by CRABPs. (A) Huh7 cells stably expressing CRABPs were infected with vesicular stomatitis virus (VSV) at the indicated PFU per milliliter for 24 h, followed by crystal violet staining. Images are representative of results from three independent experiments. (B) Cell lysates from Huh7 cells stably expressing CRABPs and infected with Sendai virus (SeV) at 100 hemagglutinating units (HAU)/ml for 24 h were subjected to immunoblot analysis for the detection of the indicated proteins. (C) Cell lysates from Huh7 cells stably expressing CRABPs and infected with dengue virus (DENV) at an MOI of 0.2 for 72 h were subjected to assessment of viral genome abundance by RT-qPCR. NS, not significant. (D) Huh7 cells stably expressing CRABPs were transfected with the p1.3x HBV DNA-encoding plasmid (HBV) or pUC19 (vector control) for 48 h, followed by immunoblot analysis for the detection of HBV core proteins. (E) Total RNAs extracted from Huh7 cells stably expressing CRABPs and infected with hepatitis E virus (HEV) at 6 × 10⁸ genome equivalents (GE)/ml for 5 days were subjected to a probe-based RT-qPCR assay for the quantification of HEV genome copies. Each bar represents the mean ± SD from triplicate samples. NS, not significant. See also Fig. S3 in the supplemental material.