FIG 5.

Mutations in M153R leading to reading frame disruptions. (A) Partial nucleotide sequence of M153R from SLS, Bluegums (BGM), Monarto Zoo (MON), and ABC Range (ABC). The early stop codon formed by 3 nucleotide substitutions in Bluegums is underlined and shaded. The A deletion in Monarto Zoo is shaded, and the G insertion in ABC Range is shaded. (B) Amino acid sequences of M153 for the same viruses described in the legend to panel A. The RING-CH E2 binding site at the N terminus is shaded, the two transmembrane domains are underlined, and the C-terminal conserved acidic domain is shaded. Lowercase amino acids in Monarto Zoo and ABC Range indicate an altered amino acid sequence following the indel. Virus names have been abbreviated for ease of presentation.