FIG 6.

Arbidol impairs LASV GP1 dissociation from GP2. (A) Flag-tagged LASV GP from cell lysates of Lamp1 KO HEK293T/17 cells was immobilized on anti-Flag (M2) magnetic beads, which were then treated with 0 or 40 μM arbidol for 1 h at 4°C. The beads were then subjected to a pulse for 1 min at 37°C at the indicated pH in the presence or absence of arbidol. The extent of GP1 dissociation was then determined by Western blot analysis of GP1 in the supernatant and bead-bound fractions. Data are the averages from four experiments. Error bars represent SEMs. (B) Flag-tagged LASV GP, prepared and immobilized as for panel (A), was pretreated with 0 or 40 μM arbidol. The beads were then exposed to pH 6.5 at 37°C for the indicated time, in the presence or absence of arbidol, and the extent of GP1 dissociation was determined as for panel A. Data are the averages from five experiments. Error bars represent SEMs.