FIG 1.

Both GP1_JUNV and GP1_LASV are immunogenic in mice. (A) ELISA using serial dilutions of sera from mice immunized with GP1_JUNV and GP1_LASV. Plates were coated with the same immunogen that was produced in insect cells and that was injected into mice. (Left and middle) The response of two groups of 4 mice each that were immunized with GP1_JUNV and GP1_LASV, respectively. Colors correspond to sera from different mice. (Right) Data for a second group of 4 mice that were immunized with GP1_LASV. Bars represent average values from three independent experiments, each consisting of several technical replicates. Error bars represent standard deviations. (Insets) Analyses of the corresponding GP1_JUNV and GP1_LASV that were used for immunization using SDS-PAGE and Coomassie staining. MW, molecular weight. (B) ELISA using the indicated serum dilution series and plates coated with the GP1 domains produced in HEK293F cells. Bars represent average values from three independent experiments, each consisting of several technical replicates. Error bars represent standard deviations. For convenience, the serum dilutions used are further indicated using a serology standard format in the lower left plot.