FIG 4.

Recognition of native unfixed spike complexes by GP1-immunized mouse sera. The results of fluorescent-activated cell sorting analyses of live unfixed cells expressing the GPC_JUNV or GPC_LASV spike complexes (top row and bottom two rows, respectively) are shown. Each graph shows staining by serum from the indicated mouse in a red curve. Control staining using serum from an unimmunized mouse is shown in gray. For GPC_JUNV-bearing cells, staining with purified Ab289 is shown in cyan. For GPC_LASV-bearing cells, staining with purified Ab37.7 is shown in cyan.