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. 2019 Mar 29;10:612. doi: 10.3389/fimmu.2019.00612

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Copyright © 2019 Guzman, Pujol, Ribeca and Montoya.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of macrophages (Macs) and dendritic cells (DCs) from the bovine afferent lymph (AL, A–F) and in vitro-derived from blood monocytes (Mo, G–L). (A, G). Based on the differential expression of CD11c and MHC class II, DCs and Macs can be defined in both, the AL and in Mo cultured in vitro with GM-CSF, IL-4, and Flt3L. DCs (red) and Macs (blue) can be further differentiated based on surface expression of CD11b (B,H), CD205 (C,I), CD1b (D,J) CD14 (E,K) and CD172a (F,L). FSC/SSC plots of DCs (red) and Macs (blue) compared with other lymphocytes present in the cultures (black) (M,N). A minimum of 50,000 single/live events were analyzed. Adherent and non-adherent cells were found to have similar phenotypes separately, therefore the mixture of both of these is shown. Figure representative of cells from 6 different animals analyzed in duplicate.