Figure 3.

Phagocytosis activity of bovine afferent lymph and monocyte-derived cells. Phagocytic activity of MoMacs (A), MoDCs (B), ALMacs (C), and ALDCs (D) was assessed using pHrodo Red E. coli BioParticles. Each histogram is representative of cells from 5 different animals. Gray histograms represent background fluorescence; dashed histograms represent cells incubated with pHrodo Red E. coli bioparticles at 4°C and solid white histograms represents fluorescence of cells incubated with bioparticles at 37°C. (E) Quantification of phagocytosis for each cell type. Bars represent the mean fluorescence intensity ratios (37°C/4°C) for cells from 5 different animals. Error bars indicate standard deviations.