Figure 11.

Multiple sequence alignment of selected isolated LG domains that are non-binders of α-DG. Laminin α3 has been reported not to bind α-DG. Therefore, it is assumed that none of its tandem LG domains would be able to bind α-DG. Secondary structure elements as retrieved from the available 3D structure of human perlecan (3SH4/3SH5): β-strands, from A to N (red), α-helices (purple), calcium-coordinating amino acid positions (orange and indicated by an asterisk). Basic residues belonging to the loops neighboring the coordinated Ca²⁺ are reported in blue whereas the one belonging to the opposite side of the domain in turquoise. Code: LNA3_LG1 to LNA3_LG5 (1st to 5th module from laminin α3).