Figure 10.

CES1 expression in primary human hepatocytes.

Notes: Cells were cultured in 3-D bioreactors or 2-D cultures for up to 120 hours after starting remimazolam application. (A) Cells cultured in 3-D bioreactors were harvested after 120 hours for mRNA expression analysis. (B) Samples from 2-D cultures were taken after 24 hours, 72 hours, and 120 hours from individual wells. Expression of CES1 was normalized to that of the housekeeping gene GAPDH, and fold changes in expression levels of remimazolam-treated cultures compared to untreated control cultures of respective time points were calculated with the ΔΔCt method; n=3, means ± SD. Underlying data can be found online at http://doi.org/10.5281/zenodo.1493510 (file Gene_expression).