Table 2.

Bioreactor perfusion parameters and operation conditions

Parameters	Set value during operation
Volume of bioreactor circuit	12 mL
Volume of cell compartment	0.5 mL
Inoculated cell number	40×106
Cell concentration in the cell compartment	80×106 cells/mL
Recirculation rate	3 mL/min
Feed rate	0.5 mL/h
Gas-flow rate	5 mL/min
Concentration of CO in supplied air/CO2 mixture2	4%–5%a
pH value	7.35–7.45
Temperature in bioreactor chamber	37°C

Note:

^aThe CO₂ concentration was adjusted on demand to maintain constant pH in the system.