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. 2019 Mar 29;10:611. doi: 10.3389/fimmu.2019.00611

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Copyright © 2019 Tan, Esterbauer, Vanderven, Juno, Kent and Wheatley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of Tfh cells in iBALT. (A) Frequency of Tfh cells (CD3+ CD4+ CXCR5++ PD1++) in MLN and lungs of mice at d35 and d56 post-infection with PR8. (B) Intracellular BCL6 staining measured by mean fluorescence intensities in Tfh and non-Tfh CD4 T cells (CD3+ CD4+ CXCR5- PD1-) isolated from MLN and lungs. (C) Frequency of proliferation marker Ki67 expressed in Tfh and non-Tfh CD4 T cells isolated from MLN and lungs. Data represent a single experiment (n = 4–5). Error bars represent mean ± SD. Visualization by immunofluorescence microscopy of (D) Ki67 and (E) BCL6 expression in T and B cells of spleen and lung iBALT; scale bar −50 μM.