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. Author manuscript; available in PMC: 2019 Apr 5.
Published in final edited form as: Mol Cell Endocrinol. 2019 Jan 4;484:59–68. doi: 10.1016/j.mce.2019.01.001

Fig. 3. ERα36 knockdown inhibits the proliferation and cell cycle progression of ht-UtLM cells induced by BPA.

Fig. 3.

(A) Proliferation of ht-UtLM cells transfected with siScr or siERα36 and treated with BPA at 10−3μM for 24h as measured by the MTS assay. The bar graph shows the mean ± SE values of absorbance of 6 wells of 96-well plates for each treatment group and indicates the increased cell proliferation induced by BPA at 10−3 μM was abolished by siERα36. *P<0.05 versus controls. (B) Relative percentage of ht-UtLM cells with siScr or siERα36 in the different phases of the cell cycle following 10−3μM BPA for 24h by flow cytometry analysis. The values of cell cycle analysis represent the number of cells in the different phases of the cell cycle as a percentage of total cells observed. The data revealed there were more cells in the S phase versus the G0-G1 phase (*P<0.008) following BPA treatment; however, this effect was inhibited by siERα36. The data shown represent three independent experiments.