Figure 4.

Coronary artery and LV coronary microvascular anatomy. (A) Ex vivo epifluorescent illumination of microspheres in sequential ventricular short-axis sections illustrating normal perfusion, and focal regions of myocardial perfusion defect (MPD) (arrows). (B) CT coronary angiography in the left lateral projection illustrating lack of arterial occlusion in 2 ponatinib-treated mice with WMAs. (C) Fluorescent confocal microscopy of the LV myocardium from ponatinib-treated wild-type mice showing regions with and without WMAs and from a sham-treated mouse. Staining was performed with isolectin (green) for microvessels, Hoechst stain for nuclei (blue), and platelet CD41 immunohistochemistry (red). The bottom rows illustrate the red channel alone to better display platelets. Scale bars, 100 μm. (D) Higher-magnification image from the ponatinib-treated animal with WMA.