Figure 2.

Immunoprofiling against heterologous and heterosubtypic viruses. Animals were vaccinated 3 times with the vaccines indicated in Table 1. After 3 vaccinations, sera, nasal, and lung washes and spleens were collected to determine systemic, mucosal and cell mediated immune responses (n = 6). Some animals were challenged with heterologous (A) and heterosubtypic (B) virus to determine protection, lung viral load (n = 6) and survival (n = 6). Generated data was used as input and conditional formatting was performed in Ms Excel to plot heatmaps. Each column represents one animal. Survival is shown with different color scheme as these are different animals compared to the rest. Dark blue indicates worst survival while light blue indicates best survival. For other parameters, heatmaps range from red (lowest response) to green (best response).