FIG. 2.

Assembly of micellar siRNA and electrostatically complexed peptide nanoparticles. (A) Micellar nanoparticles were assembled by utilizing our diblock copolymer amphiphile that combines DMAEMA as the corona-forming block and a copolymer of DMAEMA, BMA, and propylacrylic acid (PAA) as the core forming block (D-b-DBP). Nanoparticle self-assembly was induced by dilution from ethanol into PBS, and siRNA was subsequently loaded by electrostatic association. (B) Electrostatically complexed peptide nanoparticles were formed by simple mixing of the anionic PPAA polymer and cationic p-HSP20 peptide in pH 8 PBS. BMA, butyl methacrylate; DMAEMA, dimethylaminoethyl methacrylate; PBS, phosphate-buffered saline; PPAA, poly(propylacrylic acid).