Figure 1.

Tamoxifen-Induced Removal of Igf2

Schematic for tamoxifen dosing in C57BL/6 mice (A). PCR for the floxed Igf2 allele at 449 bp in iKO mice (Igf2^fl/fl Cre^+/−) with no tamoxifen or in WT mice (Igf2^fl/fl Cre^−/−) or for the recombined allele at 384 bp in iKO mice (Igf2^fl/fl Cre^+/−) plus tamoxifen (B). qPCR for Igf2 mRNA in choroid plexus (C) and hippocampus (D) from samples collected 2 weeks post tamoxifen administration. See also Figure S1. Representative sections of Luxol fast blue and H&E stains at the level of the septal nuclei and the third ventricle of WT and iKO mice (E and F). Statistical significance: ^∗p < 0.05 using ANOVA and Tukey’s post hoc test, n = 5 WT and n = 12 iKO mice. Error bars represent SEM. Scale bar, 1 mm.