Figure 4.

Loss of IGF-II Abrogates Intestinal Homeostasis

Five consecutive injections of tamoxifen (75 mg/kg) were administered from day 1 to day 5 and tissues were analyzed on day 6. Body-weight curve of WT and iKO mice from day 1 to day 6 (A), n = 3 WT and n = 4 iKO mice. WT (B and D) and iKO (C and E) guts were surgically dissected (from the stomach to the colon) and the ceca were photographed at day 6. Scale bars, 6 inches for intestines and 200 μm for cecum. Igf2 mRNA (F–G′) and IGF-II protein (I–J′) levels were detected in WT (F, F′, I, and I′) and iKO (G, G′, J, and J′) intestine using in situ hybridization and immunohistochemistry, respectively. Scale bars, 200 μm. n = 3 WT and n = 3 iKO mice. ^∗p < 0.05 by Student's t test. Error bars represent SEM.