Figure 5.

IGF-II Maintains the Morphology and Stemness of the Small Intestine

Small intestine of WT and iKO mice after five consecutive injections of tamoxifen (75 mg/kg) were stained and quantified for H&E (A–B′), Olfm4 (C–E), Ki67 (F–H), and lysozyme (I–K). qPCR analyses were performed in WT and iKO intestines to detect marker genes for stem cells (L), progenitors (M), Paneth cells (N), and quiescent stem cells (O). n = 3 (WT) and 3 (iKO) mice. See also Figure S5. Scale bars, 200 μm. ^∗ p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 by Student's t test. Error bars represent SEM.