Table 1. Macromolecule-production information.

CdAppA
Source organism	C. difficile
DNA source	C. difficile 630 chromosomal DNA
Forward primer F_CdAppA†	ttctgttccagggaccagcaGGAGCTTTTGCAAACGTTAAAG
Reverse primer R_CdAppA†	atatgtgaggagaaggcgcgTTATTCTACATACAGTTTAGACCAA
Expression vector	pET-YSBLLIC3C
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced	MGSHHHHHHSSGLEVLFQGPAGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSNLKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTASESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNADYFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEERLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTEEGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEIVPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDALWEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAILFEDWSKLYVE
CdOppA
Source organism	C. difficile
DNA source	Synthetic coding sequence codon-optimized for E. coli expression
Forward primer F_CdOppA†	ttctgttccagggaccagcaAGCAGCGGTGGCGACAAG
Reverse primer R_CdOppA†	atatgtgaggagaaggcgcgTTACTCAATGGTCCAATCCGCG
Expression vector	pET-YSBLLIC3C
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced	MGSHHHHHHSSGLEVLFQGPASSGGDKDKKADTPKDGKVLVYGSNDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDVKWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAMLDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEPGLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGILYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEKFEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKEIGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKILQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHHGVGIFWNIADWTIE

^†The upper-case sequence is complementary to the template DNA and the lower-case flanking sequence is complementary to the vector DNA for use in HiFi cloning.