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. 2019 Mar 13;75(Pt 4):246–253. doi: 10.1107/S2053230X1900178X

Table 2. Crystallization.

Method Sitting drop
Plate type 96-well MRC/Wilden
Temperature (K) 291
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 50 mM Tris–HCl pH 8.0, 150 mM NaCl
Composition of reservoir solution 0.2 M sodium iodide, 0.1 M bis-Tris propane, 20% PEG 3350
Volume and ratio of drop 150 nl, 1:1
Volume of reservoir (µl) 100