Table 2. Crystallization.
Method | Sitting drop |
Plate type | 96-well MRC/Wilden |
Temperature (K) | 291 |
Protein concentration (mg ml−1) | 20 |
Buffer composition of protein solution | 50 mM Tris–HCl pH 8.0, 150 mM NaCl |
Composition of reservoir solution | 0.2 M sodium iodide, 0.1 M bis-Tris propane, 20% PEG 3350 |
Volume and ratio of drop | 150 nl, 1:1 |
Volume of reservoir (µl) | 100 |