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Oligomeric state of Syn6301-PRK. (a) SEC-MALS analysis of thiol-oxidized Syn6301-PRK. The chromatographic absorbance trace at 280 nm wavelength is shown. For the protein peak, the molecular mass determined by static light scattering is indicated. (b) Native MS spectrum of thiol-oxidized Syn6301-PRK. The charge states of the peaks are indicated. (c) Native MS spectrum of Syn6301-PRK after the reduction of disulfide bonds by treatment with 20 mM DTT for 1 h.
