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. 2019 Apr 2;75(Pt 4):278–289. doi: 10.1107/S2053230X19002693

Table 2. Crystallization.

  Native Osmate derivative
Method Sitting-drop vapour diffusion Sitting-drop vapour diffusion
Plate type 96-well 2-drop MRC Crystallization Plates (Swissci) UVB 96-well 2-drop MRC Crystallization Plates (Swissci) UVB
Temperature (K) 277 277
Protein concentration (mg ml−1) 23 23
Buffer composition of protein solution 10 mM Tris pH 8.0, 50 mM NaCl 10 mM Tris pH 8.0, 50 mM NaCl
Composition of reservoir solution 20% PEG 3350, 200 mM ammonium fluoride 20% PEG 3350, 200 mM ammonium formate
Volume and ratio of drop 200 nl (1:1) 200 nl (1:1)
Volume of reservoir (µl) 150  150