Method |
Sitting-drop vapour diffusion |
Sitting-drop vapour diffusion |
Plate type |
96-well 2-drop MRC Crystallization Plates (Swissci) UVB |
96-well 2-drop MRC Crystallization Plates (Swissci) UVB |
Temperature (K) |
277 |
277 |
Protein concentration (mg ml−1) |
23 |
23 |
Buffer composition of protein solution |
10 mM Tris pH 8.0, 50 mM NaCl |
10 mM Tris pH 8.0, 50 mM NaCl |
Composition of reservoir solution |
20% PEG 3350, 200 mM ammonium fluoride |
20% PEG 3350, 200 mM ammonium formate |
Volume and ratio of drop |
200 nl (1:1) |
200 nl (1:1) |
Volume of reservoir (µl) |
150 |
150 |