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. 2019 Apr 2;75(Pt 4):299–306. doi: 10.1107/S2053230X19002863

Table 2. Crystallization.

Method Sitting-drop vapor diffusion
Plate type 96-well (Greiner)
Temperature (K) 299
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 10 mM Tris–HCl pH 7.5, 0.5 mM magnesium chloride, 0.5 mM L-Ala-SA
Composition of reservoir solution 0.1 M Tris–HCl pH 8.5, 0.2 M magnesium chloride, 30% PEG 4000
Volume and ratio of drop 1 µl protein solution + 1 µl reservoir solution
Volume of reservoir (µl) 50