Table 1. Protein cloning and expression.

Source organism	T. thioreducens
Forward primer	5′-TTTGTTTAACTTTAAGAAGGAGATATACATATGTTCTGGCTGAAAACCCG-3′
Reverse primer	5′-CTTCCTTTCGGGCTTTGTTAGCAGCCGGATCCTCATTCTCCCCTGTATGCCTTG-3′
Expression vector	pET-3a (Novagen) containing NdeI/BamHI insertion sites
Genome accession code	CP015105
Host	E. coli DH5 cells
Amino-acid sequence	MFWLKTRIIEGEGSLSRLSREVKGHERVLILASGSMKRHGFLSEAEDYVKEAGAEVFSIAGLPAEPSVEVIEEFLPKVREFGPDLLVAMGGGSVIDTTKALKVFYDAPELNFGEIAFIDRFSKPKPVPRLKTLLIAIPSTSGAGSEVSGASVLKKGGVKYNIVTPEIAPDVAILDPRLPRTMPPEVARNSGLDVLVHGIEAYTTKVASPFSDAMAIKAIKTVYRWLPLSVKGDEEARARVHYAATMAGIAFLNARLGLCHAMSHKAAWIGPHGLLNAVFLPYVMEFNASKSDYARRRYAEIARELGFQTAKDLIEVVKELNEMLGVPKLGELVDEETFASKVEEMAEKTYHDGLIAFNPVEPKPEEIKELYLKAYRGE
NCBI protein ID	ASJ12775