Table 2. Crystallization.

Crystal form	Monoclinic	Orthorhombic	Tetragonal
Method	Vapor diffusion	Vapor diffusion	Vapor diffusion
Plate	Cryschem	Cryschem	Cryschem
Temperature (K)	293	293	293
Protein concentration (mg ml−1)	20	20	20
Protein buffer	Water	Water	Water
Reservoir solution	8% PEG 4000 pH 4.5	8% PEG 4000 pH 4.5	0.4 M NH4H2PO4 pH 4.2
Protein:reservoir ratio	2:1	2:1	1:1
Reservoir volume (ml)	0.6	0.6	0.6
Cryoprotectant	None	None	20% PEG 3350 + 30% MPD in reservoir solution