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. 2019 Mar 13;75(Pt 4):239–245. doi: 10.1107/S2053230X19001419

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© International Union of Crystallography 2019

Prior permission is not required to reproduce short quotations, tables and figures from this article, provided the original authors and source are cited. For more information, see http://journals.iucr.org/services/copyrightpolicy.html.

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Characterization of glycosylated and nonglycosylated furin protein. Glycosylated aa108–574-Tev-FLAG-His₆ furin (circles) and nonglycosylated aa108–574-TEV-FLAG-His₆ N387D/N440D furin (squares). (a) Comparison of furin activity progress curves for glycosylated and nonglycosylated furin using the FAM-TAMRA substrate peptide as noted in Section 2. (b) Comparison of enzymatic rate versus protein concentration.