Table 2. Crystallization.
Method | Sitting-drop vapor diffusion |
Plate type | MRC 2-drop |
Temperature (°C) | 4 |
Protein concentration (mg ml−1) | 6 |
Buffer composition of protein solution | 150 mM NaCl, 5 mM CaCl2, 10 mM HEPES pH 7.5 |
Composition of reservoir solution | 11–13% PEG 8000, 0.11–0.16 M potassium dihydrogen phosphate, 0.1 M HEPES pH 7.5 |
Volume and ratio of drop | 800 nl, 1:1 |
Volume of reservoir (µl) | 60 |