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. 2019 Mar 13;75(Pt 4):239–245. doi: 10.1107/S2053230X19001419

Table 2. Crystallization.

Method Sitting-drop vapor diffusion
Plate type MRC 2-drop
Temperature (°C) 4
Protein concentration (mg ml−1) 6
Buffer composition of protein solution 150 mM NaCl, 5 mM CaCl2, 10 mM HEPES pH 7.5
Composition of reservoir solution 11–13% PEG 8000, 0.11–0.16 M potassium dihydrogen phosphate, 0.1 M HEPES pH 7.5
Volume and ratio of drop 800 nl, 1:1
Volume of reservoir (µl) 60