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. 2019 Apr 2;75(Pt 4):254–259. doi: 10.1107/S2053230X1801840X

Table 1. Macromolecule-production information.

Source organism H. sapiens
DNA source cDNA Library, Human Mammary Gland (Takara)
Expression vector Modified pFastBac1
Expression host Sf9 insect cells
Complete amino-acid sequence of the construct produced MPVKGGTKCIKYLLFGFNFI(C)FWLAGIAVLAIGLWLRFDSQTKSIFEQETNNNNSSFYTGVYILIGAGALMMLVGFLGCCGAVQESQCMLGLFFGFLLVIFAIEIAAAIWGYSHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV

The truncated residues are underlined. To prepare mercury-derivatized crystals, we introduced a cysteine residue at Ile20, which is shown in parentheses.