Table 1. Macromolecule-production information.
Source organism | H. sapiens |
DNA source | cDNA Library, Human Mammary Gland (Takara) |
Expression vector | Modified pFastBac1 |
Expression host | Sf9 insect cells |
Complete amino-acid sequence of the construct produced† | MPVKGGTKCIKYLLFGFNFI(C)FWLAGIAVLAIGLWLRFDSQTKSIFEQETNNNNSSFYTGVYILIGAGALMMLVGFLGCCGAVQESQCMLGLFFGFLLVIFAIEIAAAIWGYSHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV |
The truncated residues are underlined. To prepare mercury-derivatized crystals, we introduced a cysteine residue at Ile20, which is shown in parentheses.