Table 2. Crystallization.
| Native CD9cryst | CD9cryst I20C | |
|---|---|---|
| Method | Lipidic cubic phase | Lipidic cubic phase |
| Plate type | 96-well plastic sandwich plate | 96-well plastic sandwich plate |
| Temperature (K) | 293 | 293 |
| Protein concentration (mg ml−1) | 15 | 15 |
| Composition of reservoir solution | 10–50 mM MOPS pH 6.5, 32–38% PEG 200 or 10–50 mM Tris pH 7.5, 32–38% PEG 200 | 10–50 mM MOPS pH 6.5, 36–38% PEG 200 or 10–100 mM Tris pH 7.5, 32–38% PEG 200 |
| Co-crystallized compound (final concentration in mixture with protein solution) | — | 2 mM methylmercury chloride |
| Volume of drop (nl) | 50 | 50 |
| Volume of reservoir (nl) | 800 | 800 |