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Enzymatic-1H-NMR coupled assay for the detection of CMP-sialic acids formation. A. Enzymatic formation of CMP-sialic acid using NmCSS. B. 1H-NMR spectra of the corresponding crude reaction mixtures for CMP-Neu5Ac, -Neu9NAz, -Neu5SydCl and -Neu9NSydCl. Protons on the C3-carbon atom of the starting sialic acid (dashed blue line) and the CMP-sialic acid product (dashed orange line).
