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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Feb 19;58(13):4281–4285. doi: 10.1002/anie.201814266

Figure 4.

Figure 4.

In-gel visualization of asialofetuin (2.4 mg/mL) incubated with human ST6Gal-I (19.5 μg/mL) or ST3Gal-IV (19.5 μg/mL) in the presence of CMP-Neu5Ac (1.2 mg/mL) or its unnatural analogs CMP-Neu9Az (10) (1.2 mg/mL) or CMP-Neu9NSydCl (9) (1.7 mg/mL), or no reagent (−). The presence of the chemical reporter was detected by fluorescence imaging (incubation with BCN-FITC (top row; λexc: trans UV)) and by Coomassie Blue stain to reveal total protein content (bottom row).