Extended Data Figure 4 ∣. Alignment of the coiled-coil 1 of the stalk region in 67 dynein heavy chains.
The sequences are oriented from N-terminus to C terminus in these alignments. Isoforms of dynein used in the alignment were grouped based on the type and the organism (cytoplasmic (cyt1), axonemal outer arm (22Sab, 22Sg), axonemal inner arm (IA) and intra-flagellar transport (cyt2) dyneins). α and β registry of the stalk coiled coils are shown on top. Stalk length is conserved among dyneins. Fully conserved proline residues at the base of MTBD that cause tilting of the stalk coiled-coils towards the MT plus-end are highlighted in green. Other residues that are conserved over 90% are highlighted in yellow.