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. 2019 Mar 22;8:e44652. doi: 10.7554/eLife.44652

Key resources table.

Reagent type
(species) or
resource		 Designation Source or
reference		 Identifiers Additional
information
Gene(Escherichia coli) MalE NA UniProt:
P0AEX9
Antibody Mouse anti-his Qiagen RRID:AB_2714179 (1:200)
Strain,
strain background (Streptococcus pneumoniae)		 D39 National Collection of Type Cultures NCTC:7466 Capsular serotype 2
Strain, strain
background
(Streptococcus
pneumoniae)		 D39 ∆psaA This paper Replacement of psaA with the Janus cassette (∆psaA::Janus)
Strain, strain background (Streptococcus pneumoniae) D39 ∆czcD This paper Replacement of czcD with the Janus cassette (∆czcD::Janus)
Strain, strain background (Streptococcus pneumoniae) D39 ΩpsaAD280N This paper Replacement of ∆psaA::Janus with psaA D280N (∆psaA::psaAD280N)
Strain, strain background (Streptococcus pneumoniae) D39 ΩpsaAD280N∆czcD This paper Replacement of ∆psaA::Janus with psaA D280N; replacement of czcD with the Janus cassette (∆psaA::psaAD280N∆czcD::Janus)
Strain, strain background
(Lactococcus lactis)		 NZ9000 NIZO food
research
Strain, strain
background
(Lactococcus lactis)		 GKW9000 DOI: 10.1038/
nsmb2929		 Lactococcus lactis NZ9000 with glnPQ gene deleted
Strain, strain
background
(Escherichia coli)		 K12 Other Provided by Tassos Economou, KU Leuven
Strain, strain
background
(Escherichia coli)		 BL 21 DE3 Other Provided by Tassos Economou, KU Leuven
Recombinant
DNA reagent		 pET20b Merck Cat#:69739–3
Recombinant
DNA reagent		 pNZglnPQhis DOI: 10.1047/
jbc.M500522200		 Expression plasmid for GlnPQ
Recombinant
DNA reagent		 SBD1-T159C/G87C DOI: 10.1038/
nsmb2929		 Expression plasmid for SBD1(T159C/G87C)
Recombinant
DNA reagent		 SBD2-T369C/S451C DOI: 10.1038/
nsmb2929		 Expression plasmid for SBD2(T369C/S451C)
Recombinant
DNA reagent		 pCAMcLIC01-PsaA DOI: 10.1038/
nchembio.1382		 Expression plasmid for PsaA
Recombinant
DNA reagent		 pCAMcLIC01-PsaAD280N DOI: 10.1038/
nchembio.1382		 Expression plasmid for PsaA(D280N)
Recombinant
DNA reagent		 pNZOpuCHis DOI: 10.1093/
emboj/cdg581		 Expression plasmid for OpuAC
Recombinant
DNA reagent		 pNZcLIC-OppA DOI: 10.1002/pro.97 Expression plasmid for OppA
Recombinant
DNA reagent		 PsaA-V76C/K237C This paper Expression plasmid for PsaA(V76C/K237C) from the pCAMcLIC01-PsaA construct
Recombinant
DNA reagent		 PsaA-E74C/K237C This paper Expression plasmid for PsaA(E74C/K237C) from the pCAMcLIC01-PsaA construct
Recombinant
DNA reagent		 PsaA-D280N/V76C/K237C This paper Expression plasmid for PsaA(D280N/V76C/K237C) from the pCAMcLIC01-PsaAD280N construct
Recombinant
DNA reagent		 MalE-T36C/S352C This paper Progenitors: PCR, E. coli gDNA; pET20b vector
Recombinant
DNA reagent		 MalE-T36C/N205C This paper Progenitors: PCR, E. coli gDNA; pET20b vector
Recombinant
DNA reagent		 MalE-K34C/
R354C		 This paper Progenitors: PCR, E. coli gDNA; pET20b vector
Recombinant
DNA reagent		 MalE-T36C/S352C/
A96W/I329W		 This paper Progenitors: PCR,
E. coli gDNA;
pET20b vector
Recombinant
DNA reagent		 OpuAC-V360C/
N423C		 This paper Expression plasmid
for OpuAC(V360C/N423C)
from the pNZOpuCHis
construct
Recombinant
DNA reagent		 OppA-A209C/
S441C		 This paper Expression plasmid
for OppA(A209C/
S441C) from the
pNZcLIC-OppA
construct
Sequence-
based reagent		 Primers Merck see Supplementary File 2
Peptide,
recombinant
protein		 RPPGFSPFR Merck Cat#:B3259 peptide sequence:
RPPGFSPFR
Peptide,
recombinant
protein		 RDMPIQAF CASLO ApS peptide sequence:
RDMPIQAF
Peptide,
recombinant
protein		 SLSQSKVLPVPQ CASLO ApS peptide sequence:
SLSQSKVLPVPQ
Peptide,
recombinant
protein		 SLSQSKVLP CASLO ApS peptide sequence:
SLSQSKVLP
Chemical
compound,
drug		 Glycine Betaine Merck Cat#:B3501
Chemical
compound,
drug		 Carnitine Merck Cat#:94954
Chemical
compound,
drug		 Maltose Merck Cat#:63418
Chemical
compound,
drug		 Maltotriose Merck Cat#:851493
Chemical
compound,
drug		 Maltotetraose Carbosynth
Limited		 Cat#:OM06979
Chemical
compound,
drug		 Maltopentaose Merck Cat#:M8128
Chemical
compound,
drug		 Maltohexaose Santa Cruz
Biotechnology		 Cat#:sc-218665
Chemical
compound,
drug		 Maltoheptaose Carbosynth
Limited		 Cat#:OM06868
Chemical
compound,
drug		 Maltodecaose Carbosynth
Limited		 Cat#:OM146832
Chemical
compound,
drug		 Maltooctaose Carbosynth
Limited		 Cat#:OM06941
Chemical
compound,
drug		 Beta
Cyclodextrin		 Merck Cat#:C4767
Chemical
compound,
drug		 Maltotetroitol Carbosynth
Limited		 Cat#:OM02796
Chemical
compound,
drug		 Maltotriitol Merck Cat#:M4295
Chemical
compound,
drug		 3H-Asparagine American
Radiolabeled
Chemicals		 Cat#:ART 0500–250 µCi
Chemical
compound,
drug		 14C-Glutamine PerkinEllmer Cat#:NEC451050UC
Chemical
compound,
drug		 14C-Histidine PerkinEllmer Cat#:NEC277E050UC
Chemical
compound,
drug		 14C-Arginine Moravek Cat#:MC 137
Chemical
compound,
drug		 3H-Lysine PerkinEllmer Cat#:NET376250UC
Chemical
compound,
drug		 Alexa555 Thermo Fisher
Scientific		 Cat#:A20346
Chemical
compound,
drug		 Alexa647 Thermo Fisher
Scientific		 Cat#:A20347
Chemical
compound,
drug		 Cy3B GE Healthcare Cat#:PA63131
Chemical
compound,
drug		 ATTO647N ATTO-TECH Cat#:AD 647 N-45
Software,
algorithm		 Dual-Channel-
Burst-Search		 DOI: 10.1021/
jp063483n
Software,
algorithm		 LabView data
acquisition		 DOI: 10.1371/journal.
pone.0175766		 Provided by
Shimon Weiss,
UCLA
Software,
algorithm		 Hidden Markov
Model		 DOI: 10.1109/
5.18626
Software,
algorithm		 Origin OriginLab RRID:SCR_002815
Software,
algorithm		 MATLAB MathWorks RRID:SCR_001622