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. 2019 Mar 22;8:e44652. doi: 10.7554/eLife.44652

Table 2. Steady-state anisotropy values.

Anisotropy
Alexa555 Alexa647 Cy3B Atto647N
Free dye 0.25 0.20 0.08 0.08
OpuAC(V360C/N423C) NA NA 0.17 0.11
OppA(A209C/S441C) 0.25 0.19 NA NA
SBD1(G87C/T159C) 0.27 0.19 NA NA
SBD2(T369C/S451) 0.26 0.20 NA NA
MalE(T36C/S352C) 0.29 0.24 NA NA
PsaA(V76C/K237C) 0.28 0.22 NA NA

NA: not applicable. Data correspond to mean (s.d. below < 0.01) of duplicate experiments, using the same labeled protein sample.