Table 2. Steady-state anisotropy values.
Anisotropy | ||||
---|---|---|---|---|
Alexa555 | Alexa647 | Cy3B | Atto647N | |
Free dye | 0.25 | 0.20 | 0.08 | 0.08 |
OpuAC(V360C/N423C) | NA | NA | 0.17 | 0.11 |
OppA(A209C/S441C) | 0.25 | 0.19 | NA | NA |
SBD1(G87C/T159C) | 0.27 | 0.19 | NA | NA |
SBD2(T369C/S451) | 0.26 | 0.20 | NA | NA |
MalE(T36C/S352C) | 0.29 | 0.24 | NA | NA |
PsaA(V76C/K237C) | 0.28 | 0.22 | NA | NA |
NA: not applicable. Data correspond to mean (s.d. below < 0.01) of duplicate experiments, using the same labeled protein sample.