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. 2019 Apr 5;8:e42434. doi: 10.7554/eLife.42434

Figure 3. Cell shape changes of migrating mesoderm.

Figure 3.

(a) Cell shape progression of nascent mesoderm delaminating at the primitive streak of a Mid Streak embryo (Z-projection of two-photon stack, scale bar: 10 μm). (b) Mesoderm cells extend filopodia (arrows) towards epiblast and visceral endoderm. Embryo is at Late Streak stage. (Z-projection of two-photon stack, scale bar: 10 μm). (b’) Quantification of filopodia per cell per time point as mean ± SEM, n=40 cells out of 4 Late Streak embryos for each, p<0.0001. P value was calculated using the t test. Data can be found in Figure 3—source data 1. (c) Montage of a mesoderm cell (from a Mid Streak stage embryo) displaying seeking behavior (Z-projection of two-photon stack, scale bar: 10 μm). (d) Mesoderm cells are highlighted, through manual segmentation, in red, blue and yellow to track cell behavior after division (Z-projection of confocal stacks from a Mid Streak stage embryo, anterior view, scale bar: 50 μm). mG: membrane GFP, in green; mT: membrane dtTomato, in grey.

Figure 3—source data 1. Mesoderm cells filopodia extended towards Visceral Endoderm and Epiblast.
elife-42434-fig3-data1.xlsx (11.7KB, xlsx)
DOI: 10.7554/eLife.42434.018
Figure 3—source data 2. Quantification of daughter cells trajectory.
elife-42434-fig3-data2.xlsx (166.9KB, xlsx)
DOI: 10.7554/eLife.42434.019
Figure 3—source data 3. Quantification of trajectory of cells in close proximity.
elife-42434-fig3-data3.xlsx (231.4KB, xlsx)
DOI: 10.7554/eLife.42434.020
Figure 3—source data 4. Quantification of trajectory of cells after collision.
elife-42434-fig3-data4.xlsx (82.7KB, xlsx)
DOI: 10.7554/eLife.42434.021