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. 2019 Apr 5;8:e42434. doi: 10.7554/eLife.42434

Video 12. Mesoderm migration tracking in RhoaΔmesoderm embryo.

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DOI: 10.7554/eLife.42434.040

3D snapshots of stacks from a T-Cre; mTmG; Rhoa fl/- embryo dissected at E7.25 (Mid Streak stage) and imaged using two-photon microscopy for 120 min showing highlighted cells, which are tracked throughout the time lapse. The video shows the highlighted cells first, then the original images (Membrane GFP, in green) in a looping fashion for comparison. All other cells express membrane dtTomato (grey). Anterior oblique orientation with anterior to the left (scale bar: 50 μm).