Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Protocol |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
T-Cre | PMID: 18708576 | RRID:MGI:3811072 | |
| Genetic reagent (Mus musculus) |
Sox2-Cre | PMID: 12617844; The Jackson Laboratories |
TJL: 008454 | |
| Genetic reagent (Mus musculus) |
mTmG | PMID: 17868096; The Jackson Laboratories |
TJL: 007676 | |
| Genetic reagent (Mus musculus) |
Rac1 conditional mutant |
PMID: 14564011 | RRID:MGI:3579087 | |
| Genetic reagent (Mus musculus) |
Rhoa conditional mutant |
PMID: 21209320 | ||
| Genetic reagent (Mus musculus) |
LifeAct-mEGFP | PMID: 22658956 | ||
| Antibody | Goat polyclonal anti-Pecam-1 |
R and D systems | AF3628 | IF 1/500 |
| Antibody | Rabbit polyclonal anti-Podocalyxin |
EMD Millipore | ABD27 | IF 1/200 |
| Antibody | Rat monoclonal anti-Keratin 8 |
Developmental Studies Hybridoma Bank |
TROMA-I; AB_531826 |
IF 1/100 |
| Antibody | Rabbit monoclonal anti-Vimentin |
abcam | ab 92547 | IF 1/200 |
| Antibody | Donkey polyclonal anti rabbit Alexa Fluor 488 |
Life technologies |
A21206 | IF 1/500 |
| Antibody | Goat polyclonal anti rabbit Alexa Fluor 647 |
Life technologies |
A21244 | IF 1/500 |
| Antibody | Chicken polyclonal anti rat Alexa Fluor 647 |
Life technologies |
A21472 | IF 1/500 |
| Antibody | Donkey polyclonal anti goat Alexa Fluor 647 |
Jackson | A21447 | IF 1/500 |
| Other | TRITC-Phalloidin | Invitrogen | A12380 | 1/200 |
| Other | DAPI | Sigma | D9542 | 1/1000 |
| Commercial assay or kit |
RNAscope | ACDbio | ACDbio: 322350 |