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. 2019 Mar 25;8:e45457. doi: 10.7554/eLife.45457

Figure 5. Comparative analysis of the effect of QC-01–175 at (A).

0.01 μM, (B) 0.1 μM, (C) 1 μM, and (D) 10 μM after 4 hr, 8 hr or 24 hr of treatment. Graph bars represent mean levels of total tau (TAU5) and S396 P-tau protein measured by ELISA, normalized to total μg of protein and to vehicle-treated samples ± SEM (n = 3), in A152T 6-week differentiated neurons. Student T-test for each dose/time is relative to vehicle-treated tau levels nsp> 0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Figure 5—figure supplement 1 shows concentration effect curves for QC-01–175 at 4 hr and 8 hr, for all doses tested in A152T and P301L neurons; as well as the 4 hr effect seen by western blot. Figure 5—source data 1 includes all values plotted in the main Figure and supplement. The following figure supplement is available for Figure 5.

Figure 5—source data 1. Numerical description and statistics for data presented in Figure 5 and respective supplement 1.
elife-45457-fig5-data1.xlsx (17.1KB, xlsx)
DOI: 10.7554/eLife.45457.014

Figure 5.

Figure 5—figure supplement 1. Degrader concentration and time effect on tau, in A152T and P301L neurons.

Figure 5—figure supplement 1.

(A–C) Concentration and time effect of QC-01–175 on total tau (TAU5) and S396 P-tau levels, in A152T 6-week differentiated neurons, treated for (A) a short 4 hr interval, (B) an intermediate 8 hr interval, (C) compared to 24 hr treatment. (D) Concentration effect of QC-01–175 4 hr treatment in A152T neurons, by western blot analysis of total tau (TAU5) and P-tau S396 (representative blot is shown) with mean densitometry quantification (bands corresponding to brackets)±SEM (n = 3). (F–H) Concentration and time effect of QC-01–175 on total tau (TAU5) and S396 P-tau levels, in P301L 6-week differentiated neurons treated for (F) 4 hr, (G) 8 hr and (H) 24 hr. (A-C, F-H) Data points represent mean levels of tau protein measured by ELISA, normalized to total μg of protein and to vehicle-treated ± SEM (n = 3). Student T-test between each dose and vehicle-treated tau levels nsp>0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.