Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Arch Biochem Biophys. 2018 Jun 26;653:90–96. doi: 10.1016/j.abb.2018.06.014

Fig. 4. Absorbance spectra of the purified STM-labeled and unlabeled CYP3A4 C215 mutant.

Fig. 4.

Both enzymes are predominantly in the low-spin state (418 nm peak), but the STM-labeled mutant has the larger high-spin content (a pronounced shoulder at 386 nm). The high spin content in STM-bioconjugated CYP3A4 (~20%) was estimated based on the absorbance spectra of the ligand-free (100% low-spin) and bromoergocryptine-bound protein (100% high-spin conversion) samples.