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. Author manuscript; available in PMC: 2019 Apr 5.
Published in final edited form as: Nature. 2016 Apr 13;532(7599):323–328. doi: 10.1038/nature17624

Figure 2: Leaky sinusoids are the exclusive site for cellular trafficking.

Figure 2:

a-g, (Mean ± s.e.m., n=53 BVs were analyzed for permeability measurements and n=62 BVs were analyzed for blood flow/shear rates, from three independent experiments each). P-values, two-tailed student’s t-test (***P<0.005). a, Standard deviation heat map of Rhodamine-Dextran leakage. Color scale shows pixel intensity over 30 seconds of data acquisition. Regions of interest are bordered with white line. b, Time traces of fluorescence signal. c, Average vascular permeability. d, A plot of permeability measurements as a function of vessel diameter. e-f, Average blood flow speed and shear rate. g, Representative maximum intensity images demonstrating (I) homing of adoptively transferred lineage depleted HPC (hematopoietic progenitor cell, red) and BM MNC (blue) within the BM of a nestin-GFP (green) mouse, (II) transmigrating BM MNC (circled, blue) and adherent Lin− HPC (circled, red)(Scale bars indicate 50 μm), (III) upper and lower planes of the Z-stack taken from supplementary video 9 representing the relative proximity of a nestin+ BV to a sinusoidal trafficking site (Scale bar indicates 25 μm, n=309 trafficking events were monitored and analyzed).