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. Author manuscript; available in PMC: 2019 Jun 10.
Published in final edited form as: Oncogene. 2018 Dec 10;38(14):2565–2579. doi: 10.1038/s41388-018-0617-1

Figure 3. Conditioned media from BRAF-mutant dasatinib-resistant cells promotes invasion.

Figure 3.

Conditioned media (CM) was collected from BCPAP Control and DasRes cells. (A) Invasion assays of Control cells were performed in the presence of 1% FBS control, BCPAP Control CM, or BCPAP DasRes (DR) CM. Results shown are mean ± SEM of 3 independent experiments performed in duplicate. (B) Invasion of Cal62 (RAS-mutant) Control cells was performed in the presence of BCPAP (BRAF-mutant) Control CM or DasRes (DR) CM. Quantification was performed and data was normalized to CM from Control cells, set to 1. Results shown are mean ± SEM of 3 independent experiments performed in duplicate. (C) ELISAs were performed on conditioned media (CM) from BCPAP Control or DasRes cells. The fold-change of IL-1β in DasRes (DR) CM to Control CM is shown. Results shown are average of 2 replicates ± SD. (D) BCPAP DasRes cells were treated with the indicated inhibitors for 24 h and qRT-PCR of IL-1β was performed. Fold-change of IL1β in the BCPAP DasRes cells compared to the Control cells is shown. Results shown are the mean of 3 experiments ± SD. Data was analyzed by paired t-test. DasRes cells were maintained in 2 μM dasatinib. Symbols indicate * p≤0.05, Φ p≤0.01, δ p≤0.001, and Ψ p≤0.0001.