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. Author manuscript; available in PMC: 2019 Jun 10.
Published in final edited form as: Oncogene. 2018 Dec 10;38(14):2565–2579. doi: 10.1038/s41388-018-0617-1

Figure 4. IL-1𝛽 regulates invasion through a FAK>p130Cas>c-Jun signaling axis.

Figure 4.

(A) Invasion assays of BCPAP Control and DasRes cells were performed in the presence of absence of 0.1μg/mL of IL-1𝛽 neutralizing antibody or an IgG isotype control. Quantification was performed and data from each cell line was normalized to its respective DMSO-treated control. Results shown are mean ± SEM of 3 independent experiments performed in duplicate. (B) Invasion of BCPAP Control cells was performed in the presence of either BCPAP Control conditioned media (CM) or DasRes conditioned media, treated with either DMSO or anti-IL-1𝛽. Quantification was performed and data was normalized to the appropriate conditioned media treated with DMSO. Results shown are mean ± SEM of 3 independent experiments performed in duplicate. (C) Western blot analysis was performed on BCPAP Control and DasRes cells treated with the indicated concentration of anti-IL-1𝛽 or IgG isotype control antibody. Three independent experiments were performed and a representative blot is shown. Quantification was performed and shown as the average of 3 experiments. DasRes cells were maintained in 2 μM dasatinib. δ indicates p≤0.001; Φ indicates p≤0.01.