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. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Cell Stem Cell. 2019 Mar 14;24(4):608–620.e6. doi: 10.1016/j.stem.2019.02.009

Figure 2. Loss of Ptpn21 Enhances Mobilization of Stem and Early Progenitor Cells.

Figure 2.

(A and B) PB cells freshly collected from 6–8 week-old Ptpn21−/− and Ptpn21+/+ mice were assayed by FACS to determine the frequency and number of LSK cells (n = 9 mice per genotype) (A), and subjected to CFU assays. Hematopoietic colonies were enumerated (n = 5 mice per genotype) (B). (C) Splenocytes isolated from 6–8 week-old Ptpn21−/− and Ptpn21+/+ mice were assayed by FACS to determine the absolute numbers of LSK cells (n = 9 mice per genotype) and HSCs (n = 5 mice per genotype). (D-G) Six to eight-week-old Ptpn21−/− and Ptpn21+/+ mice were administered by intraperitoneal injection with G-CSF (50 μg/day/kg body weight) or PBS for 5 consecutive days. PB was collected and assayed by multiparameter FACS analyses to determine the frequency and absolute number of LSK cells (n = 5 mice per genotype) (D). Mice were sacrificed 2 hours after the last dose of G-CSF. The frequency and absolute number of HSCs in the spleen were determined by multiparameter FACS analysis (n = 5 mice per genotype) (E). PB cells were collected and subjected to CFU assays. Hematopoietic colonies were enumerated (n = 5 mice per genotype) (F). WBC counts in the PB were monitored 0, 2, and 4 days after G-CSF administration (n = 6 mice per genotype) (G).