Skip to main content
. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Cell Stem Cell. 2019 Mar 14;24(4):608–620.e6. doi: 10.1016/j.stem.2019.02.009

Figure 4. Ptpn21 Deficiency Increases Deformability in Stem and Early Progenitor Cells.

Figure 4.

(A and B) LSK cells were sorted from Ptpn21−/− and Ptpn21+/+ mice (n = 4 per genotype). Cell adhesion to fibronectin (Fn) and transmigration were determined by adhesion assays (A) and transwell migration assays (5 μm and 3 μm pores) in the absence or presence of SDF-1 (100 ng/mL) (B). (C) Diagram of the 3D HSC motility assay system. (D) Two layers of collagen hydrogels were prepared. Lower gels contained SDF-1 (100 ng/mL). Upper gels were either soft (0.5 mg/mL collagen), intermediate (1.0 mg/mL collagen), or stiff (5.0 mg/mL collagen). HSCs sorted from Ptpn21−/− and Ptpn21+/+ mice (n = 3 per genotype) were stained with calcein (2 μM) for 30 min, seeded on the top of upper gels, and incubated in PBS in a 37 °C 5% CO2 incubator. Five hours later, 3D stack images of HSCs migrating into the collagen gels were captured using a confocal microscopy. See also Figure S4.