KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PE-Mac-1 | eBioscience | Cat# 12-0112-83 RRID: AB_2734870 |
| PE-Gr-1 | eBioscience | Cat# 12-5931-83 RRID: AB_466046 |
| eFIuor 450-Gr-1 | eBioscience | Cat# 48-5931-82 RRID: AB_1548788 |
| PE-B220 | eBioscience | Cat# 12-0452-83 RRID: AB_465672 |
| APC-B220 | eBioscience | Cat# 17-0452-82 RRID: AB_469395 |
| PE-CD3e | BD Biosciences | Cat# 553064 RRID: AB_394597 |
| APC-eFluor 780-CD3e | eBioscience | Cat# 47-0031-82 RRID: AB_11149861 |
| PE-CD45.1 | eBioscience | Cat# 12-0453-82 RRID: AB_465675 |
| FITC-CD45.2 | BD Biosciences | Cat# 553772 RRID: AB_395041 |
| Biotin-Lineage Panel antibody | BioLegend | Cat# 133307 RRID: AB_11124348 |
| PE-Cy7-Sca-1 | BD Biosciences | Cat# 558162, RRID: AB_647253 |
| APC-Fluor 780-c-Kit | eBioscience | Cat# 47-1171-82 RRID: AB_1272177 |
| PE-Cy5-CD150 | BioLegend | Cat# 115911 RRID: AB_493599 |
| PE-CD150 | eBioscience | Cat# 12-1502-80 RRID: AB_1548767 |
| Alexa Fluor 647-CD150 | BioLegend | Cat# 115918 RRID: AB_2239178 |
| FITC-CD48 | eBioscience | Cat# 11-0481-82 RRID: AB_465077 |
| PE-CD48 | BioLegend | Cat# 103405 RRID: AB_313020 |
| Alexa Fluor 647-CD34 | BD Biosciences | Cat# 560230 RRID: AB_1645200 |
| FITC-CD16/32 | BD Biosciences | Cat# 553144 RRID: AB_394659 |
| PE-Cy5-CD127 | eBioscience | Cat# 15-1271-82 RRID: AB_468793 |
| Alexa Fluor 647-CD31 | BioLegend | Cat# 102516 RRID: AB_2161029 |
| APC-CD144 | BioLegend | Cat# 138011, RRID: AB_10679039 |
| PE-CD49d | BD Biosciences | Cat# 557420 RRID: AB_396693 |
| PE-CD29 | eBioscience | Cat# 12-0291-81 RRID: AB_657733 |
| APC-CD44 | BioLegend | Cat# 103012 RRID: AB_312963 |
| FITC-Annexin V | BioLegend | Cat# 640945 RRID: AB_2629519 |
| Alexa Fluor 488-Ki-67 | BioLegend | Cat# 151204 RRID: AB_2566800 |
| Biotin-Ki-67 | eBioscience | Cat# 13-5698-82 RRID: AB_2572794 |
| Mouse anti-Nestin | Millipore | Cat# MAB353 RRID: AB_94911 |
| Rabbit anti-PTPN21 | Abcam | Cat# Ab133812 RRID: N/A |
| Mouse anti-Septin1 | Santa Cruz | Cat# sc-398586 RRID: N/A |
| Mouse anti-phosphotyrosine | Millipore | Cat# 05-321 RRID: AB_309678 |
| Rabbit anti-phospho-Src (Tyr416) | Cell Signaling | Cat# 2101 RRID: AB_331697 |
| Mouse anti-c-Src | Santa Cruz | Cat# sc-8056 RRID: AB_627306 |
| Mouse anti-phospho-STAT5A/STAT5B (Tyr694/Tyr699) | Millipore | Cat# 05-495 RRID: AB_309762 |
| Rabbit anti-STAT5 | Santa Cruz | Cat# sc-835 RRID: AB_632446 |
| Mouse anti-phospho-ERK | Santa Cruz | Cat# sc-7383 RRID: AB_627545 |
| Mouse anti-ERK1/2 | Santa Cruz | Cat# sc-514302 RRID: AB_2571739 |
| Alexa Fluor 488- phalloidin antibody | Thermo Fisher | Cat# A12379 RRID: AB_2315147 |
| Rhodamine- phalloidin antibody | Thermo Fisher | Cat# R415 RRID: AB_2572408 |
| Mouse anti-β-actin | Santa Cruz | Cat# sc-47778 RRID: AB_626632 |
| Bacterial and Virus Strains | ||
| pReciever-Lv215 | Genecopoeia | N/A |
| pReciever-Lv215-Ptpn21 | Generated in this study | N/A |
| pReciever-Lv215- Ptpn21Δ1-307 | Generated in this study | N/A |
| pReciever-Lv215- Ptpn21C1110S | Generated in this study | N/A |
| pJG-IRES-GFP | Emory Integrated Genomics Core |
N/A |
| pJG-IRES-GFP-Septin1 | Generated in this study | N/A |
| pJG-IRES-GFP-Septin1Y246F | Generated in this study | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| IMDM culture medium | Thermo Fisher | Cat# 12440053 |
| StemSpan Serum-Free Expansion Medium (SFEM) | STEMCELL Technologies |
Cat # 09600 |
| Fetal bovine serum | Fisher Scientific | Cat# SH3008803IH |
| Recombinant Murine SCF | Pepro Technology | Cat# 250-03 |
| Recombinant Murine Flt3-Ligand | Pepro Technology | Cat# 250-31L |
| Recombinant Murine TPO | Pepro Technology | Cat# 315-14 |
| Recombinant Murine IL-3 | Pepro Technology | Cat# 213-13 |
| Recombinant Murine IL-6 | Pepro Technology | Cat# 216-16 |
| Recombinant Murine SDF-1α (CXCL12) | Pepro Technology | Cat# 250-20A |
| 5-Fluorouracil (5-FU) | Sigma | Cat# F6627 |
| G-CSF(Filgrastim) | Kirin-Amgen Inc. | N/A |
| Power SYBR Green PCR Master Mix | Thermo Fisher | Cat# 4367659 |
| Polybrene (Hexadimethrine bromide) | Sigma | Cat# 107689-10G |
| Target Retrieval Solution | Agilent | Cat# S169984-2 |
| Critical Commercial Assays | ||
| Quick-RNA Miniprep Kit | Zymo | Cat# R1050 |
| SuperScript First-Strand Synthesis System for RT-PCR | Invitrogen | Cat# 11904018 |
| BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit |
BD Biosciences | Cat# BDB554714 |
| Lineage Cell Depletion Kit, mouse | Miltenyi Biotec | Cat# 130-090-858 |
| Lenti-Pac™ HIV Expression Packaging Kit | Genecopoeia | Cat# LT001 |
| Experimental Models: Cell Lines | ||
| HEK293T | Cheng-Kui Qu Lab | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Ptpn21−/− mice | Generated in this study | N/A |
| Oligonucleotides used for qRT-PCR | ||
| Forward primer for mouse Ptpn21 cDNA: 5’-TCTTTGGAGACTCGGTTGCT -3’ | Synthesized in this study | N/A |
| Reverse primer for mouse Ptpn21 cDNA: 5’-CAGTGGCATCTTCTCCCTTC -3’ | Synthesized in this study | N/A |
| Forward primer for mouse Selplg-1 cDNA: 5’-GAAAGGGCTGATTGTGACCCC -3’ | Synthesized in this study | N/A |
| Reverse primer for mouse Selplg-1 cDNA: 5’-AGTAGTTCCGCACTGGGTACA -3’ | Synthesized in this study | N/A |
| Forward primer for mouse Esl-1 cDNA: 5’-CAAGATGACGGCCATCA -3’ | Synthesized in this study | N/A |
| Reverse primer for mouse Esl-1 cDNA: 5’-TTCCCAAGACGAATGCTGC -3’ | Synthesized in this study | N/A |
| Forward primer for mouse β-actin cDNA: 5’-AGAGGGAAATCGTGCGTGAC -3’ | Synthesized in this study | N/A |
| Reverse primer for mouse β-actin cDNA: 5’-CAATAGTGATGACCTGGCCGT -3’ | Synthesized in this study | N/A |
| Software and Algorithms | ||
| Flowjo v10.1 | https://www.flowjo.com/ | N/A |
| ImageJ v1.51 s | https://imagej.nih.gov/ij/ | N/A |
| Prism version 6 | Prism Graphpad | N/A |