Table 1.
Data collection, reconstruction, and model refinement statistics.
| apo DnaBC | DnaBC-dT36 | |
|---|---|---|
| Data collection and processing | ||
| Microscope | Titan Krios (U. Leeds) | Titan Krios (DLS) |
| Voltage (kV) | 300 | 300 |
| Electron dose (e/A2) | 61 | 58 |
| Dose rate (e/A2/fraction) | 1.53 | 1.45 |
| Detector | K2 Summit | K2 Summit |
| Defocus range (μm) | 1.2–2.7 | 1.2–2.7 |
| Calibrated pixel size (Å) | 1.07 | 1.05 |
| Micrographs | 3161 | 1858 |
| Total extracted particles (no.) | 551,641 | 473,888 |
| Final particle images (no.) | 104,913 | 229,097 |
| Symmetry imposed | C1 | C1 |
| Resolution (per 0.143 FSC) (Å) | 3.9 | 3.4 |
| Applied B-factor (Å2) | −158 | −125 |
| Refinement | ||
| RMS deviations | ||
| Bond lengths (Å) | 0.009 | 0.011 |
| Bond angles (°) | 1.208 | 1.288 |
| Ramachandran (%) | ||
| Outliers | 0.00 | 0.00 |
| Allowed | 12.89 | 9.49 |
| Favored | 87.11 | 90.51 |
| Rotamer outliers (%) | 0.49 | 0.54 |
| MolProbity score | 1.81 | 1.78 |
| Clashscore | 4.03 | 4.72 |