Chronic ethanol ingestion increases biochemical markers of liver damage in WT, SIRT3 KO, and SIRT5 KO mice. (A) Analysis of plasma alanine aminotransferase (ALT). (B) Liver Triglycerides. (C) Liver to body weight ratio. (D) Body weight change. (E) Food consumption. Data are presented as mean ± SEM. Statistical significance was determined by unpaired Student’s t-test between each group of mice, *=p<0.05, **= p<0.005, ***=p<0.0005, ****= p<0.0001